nifS [2017-8-11 16:0:3]

cysteine desulfurase, required for NAD biosynthesis

locus: BSU27880

pI: 6.43

mw: 43.63 kDa

protein length: 395 aa

gene length: 1185 bp

function: NAD biosynthesis

product: cysteine desulfurase

essential: no

ec: 2.8.1.7

synonyms: iscS

Genomic Context

categories

• [category|SW 2|Metabolism] → [category|SW 2.6|Additional metabolic pathways] → [category|SW 2.6.2|Biosynthesis of cofactors] → [category|SW 2.6.2.11|Biosynthesis of NAD(P)]

Gene

Coordinates: 2,849,569 → 2,850,756

The protein

Catalyzed reaction/ biological activity

• L-cysteine + [enzyme]-cysteine = L-alanine + [enzyme]-S-sulfanylcysteine (according to Swiss-Prot)

Protein family

• NifS/iscS subfamily (according to Swiss-Prot)

Paralogous protein(s)

• [protein|4F5945C3C8F18BD1B30D97CD516A06077C200B9D|YrvO], [protein|741DCFFBB8FF6A4E357EE5110EBE24412DE34244|NifZ], [protein|88ACE6B79534338F7C72F107B35A0B2384007088|SufS], [protein|5424A936643C90B3DA2CB60FD0FD42A64F2BAF09|YcbU]

[SW|Cofactors]

• pyridoxalphosphate [Pubmed|25447671]

[SW|Localization]

• cytoplasm (according to Swiss-Prot)

Expression and Regulation

Operons

• genes: [gene|A6746AE1B92A0CDCCB22B57B6DF78094BB0223A8|nifS]-[gene|510DE075EA964D22C42DEB171509F6CDA600A402|nadR]
  description: [Pubmed|8444804]

  sigma factors

  • [protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA]: sigma factor, [Pubmed|8444804], in [regulon|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA regulon]

  regulatory mechanism

  • [protein|510DE075EA964D22C42DEB171509F6CDA600A402|NadR]: repression, [Pubmed|8444804,16199587], in [regulon|510DE075EA964D22C42DEB171509F6CDA600A402|NadR regulon]

  regulation

  • repressed in the presence of nicotinic acid ([protein|search|NadR]) [Pubmed|8444804,16199587]

References

Reviews

• 25447671

Original publications

• 8444804,16199587,16199587,14567704